Study of the biological interactions of different food components

Abstract

Flavonoids are a group of chemical molecules found in plants, characterized by the presence of several phenolic units. Flavonoids posses strong antioxidant and anticancer properties. These molecules can act as scavengers of reactive oxygen species (ROS) and as inhibitors of various active enzymes. Thus, the aim of this study was to examine the in vitro cytotoxic properties of flavanols (catechin epicatechin, EGCG)-a group of flavonoids- and their activity as in vivo inhibitors of oxidative stress caused by ozone administration on healthy Wistar rats. Methodology: For the in vitro studies, Trypan Blue exclusion assay and MTT assay were used for cell viability and cell proliferation, respectively, of LMS, MCF-7 and MRC-5 cells. Flow cytometry was used for the determination of apoptosis/necrosis on LMS cells. For the in vivo studies, female Wistar rats aged 3-6 months were used. Oxidative stress was induced by intra-peritoneal administration of 6.1μg/kg body weight of ozone. Flavanols mix ...
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DOI
10.12681/eadd/22145
Handle URL
http://hdl.handle.net/10442/hedi/22145
ND
22145
Alternative title
Μελέτη των βιολογικών επιδράσεων συστατικών διατροφικών προϊόντων
Author
Simos, Yannis (Father's name: V.)
Date
2010
Degree Grantor
University of Ioannina
Committee members
Ευαγγέλου Άγγελος
Χαραλαμπόπουλος Κωνσταντίνος
Καρκαμπούνας Σπυρίδων
Ανωγειανάκις Γεώργιος
Καλφακάκου Βασιλική
Αλμπάνης Τριαντάφυλλος
Στεφάνου Δημήτριος
Discipline
Medical and Health SciencesBasic Medicine
Keywords
Antioxidants; Oxidative stress; Flavonoids; Catechins; Total antioxidant capacity; Antioxidant enzymes
Country
Greece
Language
Greek
Description
171 σ., im., ind.
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