Αλληλούχιση του RNA και ανάλυση της γονιδιακής έκφρασης των αιμοποιητικών στελεχιαίων κυττάρων στον συστηματικό ερυθηματώδη λύκο

Περίληψη

Ο Συστηματικός Ερυθηματώδης Λύκος (ΣΕΛ) είναι μία πρωτότυπη αυτοάνοση νόσος που επηρεάζει κυρίως τις γυναίκες και τυπικά έχει εκδηλώσεις σε πολλά όργανα, συμπεριλαμβανομένων του δέρματος, των αρθρώσεων, των νεφρών και του ν

Περίληψη σε άλλη γλώσσα

Systemic Lupus Erythematosus (SLE) is a prototypic autoimmune disease that affects mainly women and typically exhibits manifestations in multiple organs including skin, joints, kidneys and nervous system. It is a complex disease resulting from the interaction of heritable (genetic and epigenetic), hormonal and environmental factors. Immune complexes, auto-antibodies and all blood cells (auto-reactive lymphocytes, dendritic cells, etc) are involved in clinical manifestations. Hematopoietic Stem and Progenitor Cells (HSPCs) are multipotent cells giving rise to all blood cell lineages, both myeloid and lymphoid. We reasoned that the aberrancies of immune cells observed in SLE could be traced back to HSPCs. The aim of the study is to further investigate the role and function of bone marrow-derived HSPCs in SLE. Bone marrow (BM) samples from female NBZW/F1 lupus-prone mice and their age-matched controls were used. HSPCs defined as Lin-Sca-1+c-Kit+ hematopoietic progenitors (LSK). Transcrip ...