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Beneficial effects of sildenafil on tissue perfusion and proinflammatory molecules expression in a murine model of hind limb ischemia and atherosclerosis.Background: Sildenafil, a phosphodiesterase type 5 antagonist has endothelium protective and angiogenic effects. We tested the hypothesis that sildenafil might improve tissue perfusion and neovascularization and downregulate proinflammatory molecules following limb ischemia.Methods: 30 ApoE-/- male mice,10 weeks old, bred with cholesterol rich diet for 4 weeks, were anesthetized and underwent sunilateral hind-limb ischemia with ligation of the left femoral artery. Mice were randomized in two groups and received sildenafil (1mg/Kg for 7 days in 0.4ml solution, intraperitoneally i.p.), or normal saline (0.4ml for 7days, i.p.). Bilateral hind-limb perfusion was estimated by laser Doppler perfusion imaging after surgery on days 1, 7 and 28. sICAM-1, sVCAM-1, MMP-9, sE-Selectin and tPAI-1 levels were evaluated at days 1 and 28 with enzyme- ...
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